Description
Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy, mainly because of its high sensitivity and high specificity. Fluorescence is a form of luminescence. Fluorescence microscopy even allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations, as well as intra- and intercellular processes like endocytosis and exocytosis, can be observed. With the help of super-resolution fluorescence microscopy, it is even possible to image sub-resolution structures.
