GKE-5011B- AdvanceBio PNGase A (almond), 10 mU (formerly Prozyme). Enzyme cleaves N-linked glycans from glycopeptides, including glycans with α(1,3)-linked core fucose as seen in insects and plants. Includes reaction buffer: 50 mM citrate-phosphate buffer, pH 5.0, 0.02% sodium azide.

Endoglycosidases

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Артикул: GKE-5011B TME арт.: GKE-5011B

Продавец

Технические характеристики

Enzyme Formulation	Lyophilized powder containing citrate-phosphate buffer, pH 5.0.
Enzyme Source	Almond. Enzyme also knows as Peptide N-Glycosidase A, Glycopeptidase from almonds, Almond glycoamidase, Peptide-N(4)-(N-acetyl- glucosaminyl) asparagine amidase.
Enzyme Specific Activity	> 5 units/mg 5 units/mg —>
Enzyme Specificity	PNGase A from almonds cleaves N-glycan chains linked to Asparagine from glycopeptides. The enzyme hydrolyzes an N(4)-(acetyl-ß D-glucosaminyl) asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-ß D-glucosaminylamine and a peptide containing an aspartate residue. The enzyme cleaves N-glycans with or without a(1,3)-linked core fucose residues present in insect and plant glycoproteins. This differs from PNGase F, which cannot remove N-glycans with a(1,3)-linked core fucose. PNGase A requires glycopeptides as substrates. Glycopeptides can be obtained by digesting the glycoprotein with trypsin [1,2] or pepsin. Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino acid residues in the substrate.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to hydrolyze 1 mol ovalbumin glycopeptide per min at pH 5.0 and 37C.
Molecular Weight	54.2 and 21.2 kDa
Unit	10 mU
pH Optimum	5
pH Range	4-6