GK80021- AdvanceBio Sialidase S, His-tagged (formerly ProZyme). Enzyme releases α(2-3)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Replaces Sialidase S (GK80020). Includes 5x reaction buffer B [250 mM sodium phosphate pH 6.0].

Технические характеристики

Enzyme Formulation	Supplied lyophilized. 20 mM Tris-HCl, 25 mM NaCl (pH 7.5) upon reconstitution as indicated
Enzyme Source	AdvanceBio Sialidase S. Recombinant from Streptococcus pneumoniae, expressed in E. coli. 6xHis-tagged. Sialidases are also known as neuraminidases.
Enzyme Specificity	This enzyme preferentially releases a(2-3)-linked N-acetylneuraminic acid (Neu5Ac, NANA) from complex carbohydrates. Sialidase S is also active against a(2-3)-linked N-glycolyneuraminic acid [1] (Neu5Gc, NGNA), although similarly to other sialidases [2] the activity is lower toward Neu5Gc than Neu5Ac. Although the a(2-3)-linkage is preferred, Sialidase S (also known as NanB) is also capable of cleaving a(2-6)-linked sialic acid [3], particularly at higher concentrations. Adding an excess of this enzyme can lead to some cleavage of a(2-6)-linked sialic acid. Using GK80021 as directed in the certificate of analysis reduces the likelihood of these effects. Users may wish to optimize the enzyme concentration needed for specific proteins. May be used for exoglycosidase sequencing with Sialidase A (GK80040), which has a broader specificity, releasing a(2-3,6,8,9)-linked sialic acids. Sialidases are also known as neuraminidases.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of 1 mole of p-nitrophenol from p-nitrophenyl—D-N-acetylneuraminic acid per minute at 37 C, pH 5.5
Molecular Weight	~75 kD
Unit	3 U

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