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Paraformaldehyde-Glutaraldehyde Karnovsky»s Fixative
Kit contains
- 2 x 10 ml 16% Paraformaldehyde Solution
- 1 x 10 ml 50% EM Grade Glutaraldehyde
- 1 x 50 ml Sodium Phosphate Buffer .2M, pH 7.2
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Technical Data Sheet
Mixture 1 (Original Karnovsky»s Mixture)
16% Paraformaldehyde Solution 20 ml 50% Glutaraldehyde EM Grade 8 ml 0.2M Sodium Phosphate Buffer 25 ml Distilled Water 25 ml
Final mixture is 78 ml with 5% Glutaraldehyde, 4% Formaldehyde in 0.064M buffer. This mixture is extremely hypertonic, with an osmolarity of more than 2000m OSM .
Mixture 2
A lower concentraction of Glutaraldehyde and Formaldehyde especially used for vascular profusion.
16% Paraformaldehyde Solution 13 ml 50% Glutaraldehyde EM Grade 5 ml 0.2M Sodium Phosphate Buffer 50 ml Distilled Water 32 ml
Final mixture is 2% Paraformaldehyde, 2.5% Glutaraldehyde and 0.1M Buffer. If necessary, the osmolarity can be adjusted by adding sucrose to NaCl. The pH may also need to be adjusted to your desirable point. This mixture is the most effective general fixative for both plant and animal specimens.
Технические характеристики
16% Paraformaldehyde Solution
