The T cell intracellular antigen 1 (TIA-1) is a 17 kDa cytoplasmic granule-associated protein, expressed in cells possessing cytolytic potential. TIA-1 is also designated as GMP-17 (granule membrane protein of 17 kDa). The GMP-17/TIA-1 molecule could be involved in the molecular signaling cascade during Fas (CD95)-mediated apoptosis. In normal peripheral blood lymphocytes, TIA-1 is expressed by 90% of CD16+, 50-60% of CD8+, and less than 10% of CD4+ cells. Although TIA-1 may be detected in unstimulated T lymphocytes, activated T lymphocytes are induced to express disulfide linked polymers of the 17 kDa protein, also recognized by TIA-1. TIA-1 is expressed by all monocytes and granulocytes, by activated CD4+ T-cell clones and activated natural killer cell clones, and by Con A-activated thymocytes. On the contrary, TIA-1 is not expressed by B lymphocytes or B-cell lines.
Технические характеристики
| Target Species | Human |
| Specificity | TIA-1 |
| Other Names | Cytotoxic lymphocytes, T cell intracellular antigen 1, TIA-1 |
| Fluorochrome | PE |
| Regulatory Status | ASR |
| Size | 2 mL |
| Format | Liquid |
| Flow Product Line | IOTest |
| Clone | 2G9A10F5 |
| Isotype | IgG1 Mouse |
| Clone Description | The 2G9 antibody is a useful reagent for detecting cytolytic effector cells in tissue infiltrates. It was evaluated during HLDA 5 in the section of monoclonal antibodies reactive with intracellular antigens. It may be used to detect intracytoplasmic TIA-1 in flow cytometry after permeabilization with IntraPrep permeabilization reagent. |
