Description
The iBlot 2 Gel Transfer Device is an integral part of the iBlot 2 Dry Blotting System, which consists of the transfer device and consumable transfer stacks that contain the required buffers and transfer membrane (nitrocellulose or PVDF)
- Complete protein transfer in 7 minutes or less
- High detection sensitivity and even transfer
- Increased blotting reliability and reproducibility
- Flexible gel size formats and membrane types
- A simple, user-friendly system
- Options to create new custom programs
- Built-in tutorial and application notes
- High-quality transfer stacks that are more compact than before
Rapid, High-quality Protein Transfers
- For dry electroblotting of proteins from mini-, midi-, and E-PAGE™ gels onto nitrocellulose or PVDF membranes for Western detection
- Offers high-quality transfer, convenience, and speed, producing crisp, clear bands that remain sharp and straight, with exceptional transfer efficiency
- Allows users to create custom programs
iBlot 2 Transfers Save Time
- With the iBlot 2 system, there is no need to prepare buffers, pretreat the gel, or clean up after blotting
- The total preparation and run time is normally less than 10 minutes per blot for significant time savings compared to conventional Western transfer techniques
How It Works
- Buffer ion reservoirs are incorporated into the gel matrix (transfer stacks) instead of buffer tanks or soaked papers
- The high density of ions in the gel matrix enables rapid protein transfer
- During blotting, the copper anode does not generate oxygen gas as a result of water electrolysis, reducing blot distortion (conventional protein transfer techniques, including wet, semi-wet, and semi-dry, use inert electrodes that generate oxygen)
- Transfer time is reduced by the shortened distance between electrodes, high field strength, and high current
- Trapped air bubbles, often created during the manual preparation of the blotting sandwich layers, are easy to avoid due to the de-bubbling design that promotes even and complete transfer
Proteins, Expression, Isolation and Analysis, Western Blotting
