Product Description Modular designMultiple configurations availablethis one has them all ! Simplified sample prepNo wash, no lyse options, non-clogging fluidics Enables unique applicationsComplex protocols on a broad range of cell types and samples Customized 4-laser, 14-color system Data at up to 35,000 events/second. Detect particles with diameters from 0.5 &mu,m to 50 &mu,m. The Attune NxT can be designed to accommodate most common fluorophores and fluorescent proteins used in flow cytometry to match most panels you are currently running. Multiple fluorescent proteins can be detected with an (optional) 561 nm laser. The Attune NxT flow cytometer, designed to use acoustic-assisted hydrodynamic focusing, and consequently achieves sample-throughput rates of of 12.5 L/min1 mL/min or 12.51,000 L/minup to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec. Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations. Billed as a personal flow cytometer, the Attune NxT AFC2 matches the most sophisticated multi laser multi color instruments on the market today and meets the demands of the most complex experiments. Technical Specifications Lasers Violet: 405 nM (50 mW) Blue: 488 nM (50 mW) Yellow: 561 nM (50 mW) Red: 637 nM (100 mW) Filters VL1: 440/50 VL2: 512/25 VL3: 603/48 VL4: 710/50RL1: 670/14 RL2: 720/30 RL3: 780/60YL1: 585/16 YL2: 620/15 YL3: 695/40 YL4: 780/60BL1: 530/30 BL2: 590/40 BL3: 695/40 Scatter Forward scatter: Photodiode detector with 488/10 Side scatter: PMT with 488/10 Control and Analysis Dell Desktop with Intel CORE i7 with Windows 7 Attune NxT Software version 2.2 Dell Flat Panel LCD monitor Automated maintenance startup (less than 15 min), shutdown, rinse, wash, debubble, deep clean, monthly decontamination Gates standard and customizable gates Graphics resolution publication-quality images (customizable) Probe Design Intelligent probe design minimizes clogging and carryover (less than 1%) helps prevent damage to the instrument Sample Mixing Mixes sample by aspiration to maintain sample homogeneity and cell viability Laser Simmer mode: automatic shutoff prolongs laser usage lifetime by 10xPre-aligned and welded fiber to laser interfacePre-aligned fiber to beam-shaping optics (BSO) interface (alignment-free)All lasers spatially separatedFocus lens per laser: power where you want itFlat-top laser specified at the flow cell Benefits of flat-top lasers ~50% reduction in scattered light No problems with lateral shift in alignment