GKX-5013- β(1-3,4)-Galactosidase (bovine testis) (formerly ProZyme). Enzyme hydrolyzes nonreducing terminal galactose β(1-3) and β(1-4) linkages. Can be used with other β-galactosidases for exoglycosidase sequencing. Includes 5x reaction buffer (500 mM sodium citrate/phosphate pH 4.0).

Exoglycosidases

Доставка импортных компонентов по России от 3х недель транспортными компаниями СДЭК, Деловые Линии, Major Express.

Оплата физическими и юридическими лицами безналичным расчетом.

Цена может измениться после запроса у поставщика!

Артикул: GKX-5013 TME арт.: GKX-5013

Продавец

Технические характеристики

Concentration	5 U/mL
Enzyme Applications	The enzyme has applications in the analysis of a wide variety of glycoconjugates. It is particularly useful for ensuring the complete removal of ß(1-3) and ß(1-4)-linked non-reducing terminal galactose residues of oligosaccharides. Gal ß(1-6) GlcNAc is hydrolyzed more slowly, however this linkage is not normally encountered in native complex glycans. This activity towards ß(1-3) and ß(1-4)-linked galactose contrasts with that of our other ß-galactosidases (e.g. from S. pneumoniae, GKX-5014, or Jack bean, GKX-5012) which exhibit a preference for Gal ß(1-4), and cleave the Gal ß(1-3) linkage relatively slowly, if at all. Used in conjunction, these enzymes provide a powerful means to determine linkage positions of non-reducing ß galactose residues.
Enzyme Formulation	20 mM sodium citrate phosphate, 150 mM NaCl (pH 4.0)
Enzyme Source	Bovine testis.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to hydrolyze 1 mole of pNP-ß-D-galactopyranoside per minute at pH 4.0 and 37C.
Molecular Weight	~68 kD
Unit	0.5 U
Volume	100 µL
pH Optimum	4