| Enzyme Specificity | - The enzyme has broad specificity, cleaving a(1-2,3,6)-linked mannose, although some kinetic preference has been observed (a1-2, 6 > 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37C, complete removal of all nonreducing terminal a linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a-mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme with the a- mannosidase from Xanthomonas mannihotis which rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with Jack bean a-mannosidase (GKX-5010). 3). The enzyme will not cleave a single a(1-6) linked mannose residue from core ß-mannose, but will, however, remove a single a1-3 linked mannose from the core ß-mannose. By using enzyme concentrations of around 50 U/ml and extended incubation times (up to 18 hours) at 37C, complete removal of all nonreducing terminal a linked mannose residues may be achieved. To expedite glycan sequencing studies, the sluggish activity of GKX-5010 jack bean a-mannosidase toward a(1-6)-linked mannose residues can be overcome by using the enzyme with the a- mannosidase from Xanthomonas mannihotis which rapidly cleaves a(1-6) linkages. The X. mannihotis a-mannosidase may inhibit the action of other mannosidases if a branched (a1-6) mannose is present in the substrate, so is typically added after incubation of the substrate with Jack bean a-mannosidase (GKX-5010). —>
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![GKX-5010- α(1-2,3,6)-Mannosidase (jack bean) (formerly ProZyme). Enzyme releases nonreducing terminal α(1-2,3,6)-linked mannose from oligosaccharides. Includes incubation buffer [(500 mM sodium acetate, 10 mM zinc chloride (pH 5.0)].](https://cdn.laserzz.ru/uploads/2023/10/specificity_gkx-5010.jpg)
