GKE-5020B- AdvanceBio N-Glycanase-ultra (PNGase F, EDTA free), ≥10 U/mL (formerly ProZyme). A recombinant form of PNGase F (400 mU, EDTA free), releases intact N-glycans by cleaving between the innermost GlcNAc and Asn. The same formulation provided in GlykoPrep, ≥ 10 U/mL and EDTA-free. Includes 5x N-glycanase reaction buffer, denaturation solution, detergent solution

Endoglycosidases

Доставка импортных компонентов по России от 3х недель транспортными компаниями СДЭК, Деловые Линии, Major Express.

Оплата физическими и юридическими лицами безналичным расчетом.

Цена может измениться после запроса у поставщика!

Артикул: GKE-5020B TME арт.: GKE-5020B

Продавец

Технические характеристики

Concentration	10 U/mL
Enzyme Applications	To obtain efficient deglycosylation of glycoprotein substrates under non-denaturing conditions, it is necessary to use a higher starting concentration of enzyme. N-Glycanase PLUS and ULTRA (EDTA-Free) are supplied at = 10 U/ml, and are recommended for all applications requiring deglycosylation of glycoproteins in the absence of denaturants. The high activity also allows smaller reaction volumes and shorter reaction times to be explored.
Enzyme Formulation	20 mM Tris-HCl, 50 mM NaCl (pH 7.5, EDTA-free formulation).
Enzyme Source	Recombinant gene from Elizabethkingia meningoseptica, expressed in E. coli. The source organism was previously known as Chryseobacterium [Flavobacterium] meningosepticum. Enzyme is also known as Endoglycosidase F2, endo-beta-N-acetylglucosaminidase. Enzyme also known as PNGase F, peptide-N-glycosidase F, peptide-N4-(N-acetyl—glucosaminyl)asparagine amidase.
Enzyme Specific Activity	10 units/mg
Enzyme Specificity	Cleaves all N-linked complex, hybrid or high mannose oligosaccharides, unless a(1-3) core fucosylated, as in plant glycans. Asparagine must be peptide bonded at both termini. Phosphate, sulfate, and sialic acid groups attached to the oligosaccharide do not affect cleavage. Endo F free. Highly concentrated, particularly useful for deglycosylation under native conditions.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 mole of denatured ribonuclease B per minute at 37C, pH 7.5.
Molecular Weight	~35,000 Daltons
Unit	400 mU
Volume	40 µL
pH Optimum	8.6
pH Range	7.5-9.5