GK80030- AdvanceBio Sialidase C, 1 U (formerly ProZyme). Enzyme releases α(2-3,6)-linked sialic acid from oligosaccharides, glycoproteins, complex carbohydrates. Includes 5x reaction buffer B [250 mM sodium phosphate pH 6.0].

Exoglycosidases

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Артикул: GK80030 TME арт.: GK80030

Продавец

Технические характеристики

Concentration	10 U/mL
Enzyme Formulation	20 mM Tris HCl pH 7.5, containing 25 mM NaCl
Enzyme Source	Recombinant from Clostridium perfringens, expressed in E. coli. Formerly known as product code GE24. Sialidases are also known as neuraminidases.
Enzyme Specific Activity	40 units/mg
Enzyme Specificity	This enzyme releases a2-3, and a2-6 linked N-acetylneuraminic acid from complex carbohydrates. This enzyme will not efficiently cleave NeuAc a2-6 linked to N-acetylgalactosamine (GalNAc) when the GalNAc is labeled with a fluorophore. Sialidase A (GK80040) is recommended for this application.
Enzyme Unit Definition	One unit is defined as the amount of enzyme required to catalyze the release of 1 mole of p-nitrophenol from p-nitrophenyl—D-N-acetylneuraminic acid per minute at 37C, pH 5.5
Molecular Weight	~41,000 daltons
Unit	1 U
Volume	100 µL
pH Optimum	6