Description
Includes Computer loaded w/ Image Lab Software
The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications.
Its features are based on CCD high-resolution, high-sensitivity detection technology, and modular options to accommodate a wide range of samples and support multiple detection methods. The system is controlled by Image Lab software to optimize performance for fast, integrated, and automated image capture and analysis of various samples.
Features and Benefits
- Multiple imaging capabilities — the ChemiDoc MP imager can accommodate a variety of sample types and detection methods including multiplex fluorescent western blotting. It is the perfect imager to accompany your protein and DNA electrophoresis runs as well as your western blotting experiments. It delivers quantitative, reproducible results for fluorescence, chemiluminescence, and colorimetric detection
- Stain-free technology — UV-induced fluorescence labeling of proteins in the stain-free gels allows a 2 hr Coomassie gel-staining protocol to be condensed into a 5 min stain-and-image step. Stain-free gels are western blot compatible — using the V3 Western Workflow™, check your electrophoresis results and blot transfer quality prior to western blotting
- High-sensitivity blot detection — the ChemiDoc MP imaging system offers advanced detection technology that determines optimal exposure, even for faint or intense samples. Superior sensitivity is achieved for chemiluminescence and multiplex fluorescence detection and for colorimetric gel and blot documentation
- Superior Image Quality — exceptional dynamic range enables visualization of faint and intense bands on same blot or gel. Images are always in focus at any zoom level to ensure publication-ready images in seconds
- Ease of Use — precalibrated system provides the precise focus for any zoom setting or sample height, automated hands-free operation ensures consistent, reproducible, and high-throughput performance
Applications
- Chemiluminescence: Yes
- Fluorescence: Yes
- Colorimetry/densitometry: Yes
- Gel documentation:Yes
Hardware Specifications
- Maximum sample size: 28 x 36 cm
- Maximum image area: 26 x 35 cm
- Maximum image area: (For standard UV-excited gels) 25 x 26 cm
- Excitation source: Epi-white light and trans-UV (302 nm) are standard (optional 365 nm lamp available), optional trans-white conversion screen, XcitaBlue™ conversion screen, and epi-red, epi-green, and epi-blue LED modules available
- Illumination control: 8 modes available. Trans-UV, epi-white, and no illumination for chemiluminescence are standard, epi-red, epi-green, epi-blue, trans-white, and XcitaBlue conversion screens are optional
- Detector: Supercooled CCD
- Image resolution: 4 megapixels
- Pixel size (H x V): 6.45 x 6.45 µm
- Cooling system: Peltier
- Camera cooling temperature: -30°C absolute and regulated
- Filter holder: 6 positions (5 for filters, 1 without filter for chemiluminescence)
- Emission filters: 1 included (standard), 4 optional
- Dynamic range: >,4.0 orders of magnitude
- Pixel density (gray levels): 65,535
- Dynamic flat fielding: Application specific, for all applications
- Instrument size (L x W x H): 36 x 60 x 96 cm
- Instrument weight: 32 kg
Operating Ranges
- Operating voltage: 110/115/230 VAC nominal
- Operating temperature: 10–28°C (21°C recommended)
- Operating humidity, noncondensing: <,70%
Automation Capabilities
- Workflow automated selection: Application driven, user selected or recalled by a protocol
- Workflow automated execution: Controlled by a protocol via application-specific setup for image area, illumination source, filter, analysis, and reporting
- Workflow reproducibility: 100% repeatability via recallable protocols, from image capture to quantitative analysis and reports
- Autofocus (patent pending): Precalibrated focus for any zoom setting or sample height
- Image flat fielding**: Dynamic, precalibrated and optimizor every application
- Autoexposure: 2 user-defined modes (intense or faint bands)
