AdvanceBio Gly-X deglycosylation and InstantQ labeling module set, 24-ct (formerly ProZyme). Utilizes a novel in-solution enzymatic protein deglycosylation followed by rapid labeling of released N-glycans with InstantQ dye. InstantQ dye is specifically designed for glycan separation and detection using the Gly-Q instrument and a Gly-Q InstantQ cartridge. Includes GX24-300 Gly-X deglycosylation module for InstantQ, GX24-301 Gly-X InstantQ labeling module.(For sample cleanup use GX96-302).

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Технические характеристики

Application	<!— ‘multilineKeyVal’: Flexible, high-throughput format: process 1 to 24 samples—> <!— ‘multivalue1’: Flexible, high-throughput format: process 1 to 24 samples—> <!— ‘multivaluekeyval after making as empty’: Flexible, high-throughput format: process 1 to 24 samples—> Flexible, high-throughput format: process 1 to 24 samples
Count	24
Deglycosylation Efficiency	˃ 99 %
Equipment & Reagents Provided by User	<!— ‘multilineKeyVal pipe value’:96-well Thermocycler or two independent heat blocks, set for 90 C and 50 C Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended) Formic acid, MS-grade (Fisher A117-50 recommended) THERM-1000, 230V) Thermocycler (Corning THERM-1001, 110V Vacuum manifold (Millipore MSVMHTS00-HTS) Vacuum pump (Millipore WP6211560, 110 V WP6122050, 220V Welch WOB-L Pump 2522) —> 96-well Thermocycler or two independent heat blocks, set for 90 C and 50 C Acetonitrile (ACN), MS-grade (Fisher A955-4 recommended) Formic acid, MS-grade (Fisher A117-50 recommended) THERM-1000, 230V) Thermocycler (Corning THERM-1001, 110V Vacuum manifold (Millipore MSVMHTS00-HTS) Vacuum pump (Millipore WP6211560, 110 V WP6122050, 220V Welch WOB-L Pump 2522)
Instrument Compatibility	Gly-Q Glycan Analysis System
Kit Configuration	Sample Prep Module Set
Label	InstantQ
Precision (well-to-well, day-to-day)	<!— Precision (well-to-well, day-to-day)::
Prep time (approx)	30 min (12 samples), 90 min (96 samples)
Preservation of Sialylated Species	> 95 % 95 % —>
Protein Load Range	1-40 µg
Recovery	> 85 % 85 % —>
Stability	Up to 18 hrs on RT autosampler, and -20C for up to 30 days in water
Technique	CE