| Concentration | |
| Incubation Time | - Incubate 30 minutes at 37 °C
|
| Number of Reactions | |
| Reaction Conditions | - 40mM Tris-HCl (pH 8.0), 8 mM MgCl2, 50 mM NaCl, 2 mM spermidine, 30 mM DTT, 400 M of each rNTP, 1 g DNA template and 10 units enzyme in a 25 L volume. Incubate 30 minutes at 37C
|
| Storage Buffer | - 20 mM KPO4 (pH 7.7), 100 mM NaCl, 10 mM DTT, 1 mM EDTA, and 50 % glycerol (v/v)
|
| Unit Definition | - One unit will catalize the incorporation of 1.0 nmole of nucleoside triphosphate into an acid insoluble form in 60 minutes at 37C
|